Isolation and differentiation of mesenchymal stem cells from bovine umbilical cord blood.
Reprod Domest Anim
; 46(1): 95-9, 2011 Feb.
Article
en En
| MEDLINE
| ID: mdl-20345587
ABSTRACT
Currently, mesenchymal stem cells (MSCs) are used in veterinary clinical applications. Bone marrow and adipose tissue are the most common sources of stem cells derived from adult animals. However, cord blood which is collected non-invasively is an alternative source of stem cells other than bone marrow and adipose tissue. Moreover, high availability and lower immunogenicity of umbilical cord blood (UCB) haematopoietic stem cells compared to other sources of stem cell therapy such as bone marrow have made them a considerable source for cell therapy, but MSCs is not highly available in cord blood and their immunogenicity is poorly understood. In this study, the cells with spindle morphology from 7 of 9 bovine UCB samples were isolated and cultured. These mesenchymal stromal cells were successfully differentiated to osteocytes, chondrocytes and adipocytes. In addition, Oct-4 and SH3 were determined by RT-PCR assay. It is the first report of isolation, culture, characterization and differentiation of bovine umbilical stem cells.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bovinos
/
Diferenciación Celular
/
Separación Celular
/
Sangre Fetal
/
Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
Reprod Domest Anim
Asunto de la revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Irán