Hb Tak and Hb Q-Thailand in Thai patients are S-window hemoglobin variants revealed by high performance liquid chromatography.
Hemoglobin
; 34(2): 161-4, 2010 Jan.
Article
en En
| MEDLINE
| ID: mdl-20353352
ABSTRACT
The S-window hemoglobin (Hb) variants revealed by high performance liquid chromatography (HPLC) were studied in 12 Thai individuals. The variants were identified, using DNA sequencing and multiplex amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), to be six cases of Hb Tak [beta147 (+AC)], and six cases of Hb Q-Thailand [alpha74(EF3)Asp-->His], respectively. By using the Capillarys 2-capillary zone electrophoresis (CE), Hb Tak and Hb Q-Thailand co-migrated with Hb F in zone 7. This might pose a problem as the high Hb F conditions suggest a differential diagnosis. The S-window Hb variants are mostly Hb Tak and Hb Q-Thailand in the Thai population rather than Hb S [beta6(A3)Glu-->Val]. The definite identification of Hb variants detected by HPLC or capillary electrophoresis (CE) requires DNA analysis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Electroforesis de las Proteínas Sanguíneas
/
Hemoglobinas Anormales
/
Cromatografía Líquida de Alta Presión
/
Electroforesis Capilar
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Hemoglobin
Año:
2010
Tipo del documento:
Article
País de afiliación:
Tailandia