Characterization of interactions between proteins using site-directed spin labeling and electron paramagnetic resonance spectroscopy.
Methods Mol Biol
; 619: 173-90, 2010.
Article
en En
| MEDLINE
| ID: mdl-20419411
ABSTRACT
Site-directed spin-labeling and the analysis of proteins by electron paramagnetic resonance spectroscopy provides a powerful tool for identifying sites of contact within protein complexes at the resolution of aminoacyl side chains. Here we describe the method as we have used it to study interactions of proteins involved in export via the Sec secretory system in Escherichia coli. The method is amendable to the study of most protein interactions.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Marcadores de Spin
/
Proteínas
/
Espectroscopía de Resonancia por Spin del Electrón
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos