Simplified capillary electrophoresis nanospray sheath-flow interface for high efficiency and sensitive peptide analysis.
Rapid Commun Mass Spectrom
; 24(17): 2554-60, 2010 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-20740530
ABSTRACT
We report a simple nanospray sheath-flow interface for capillary electrophoresis. This interface relies on electrokinetic flow to drive both the separation and the electrospray; no mechanical pump is used for the sheath flow. This system was interfaced with an LCQ mass spectrometer. The best results were observed with a 2-microm diameter emitter tip and a 1-mm spacing between the separation capillary tip and the emitter tip. Under these conditions, mass detection limits (3sigma) of 100 amol were obtained for insulin receptor fragment 1142-1153. The separation efficiency exceeded 200,000 plates for this compound. The relative standard deviation generated during continual infusion of a 50 microM solution of angiotensin II was 2% for the total ion count and 3% for the extracted ion count over a 40-min period. Finally, the interface was also demonstrated for negative ion mode.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Péptidos
/
Electroforesis Capilar
/
Espectrometría de Masa por Ionización de Electrospray
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Rapid Commun Mass Spectrom
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos