Differential development of plasmacytoid dendritic cells in Th1- and Th2-like cytokine milieus.
Allergy
; 66(3): 386-95, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21039603
ABSTRACT
BACKGROUND:
Plasmacytoid dendritic cells (pDCs) infiltrate sites of Th1- and Th2-dominant inflammation and many studies have been performed to analyse their role in these immune responses. In contrast, much less is known about the effects of a Th1 or Th2 cytokine milieu on pDC function. Therefore, we investigated the impact of Th1- and Th2-like conditions during the development of pDCs on their antigen expression and function.METHODS:
PDCs were matured in vitro by the addition of IL-3 under Th1- or Th2-like conditions. Antigen expression and TLR7-ligand-induced cytokine secretion was analysed by flow cytometry and ELISA. Furthermore, the CD4(+) T-cell polarizing capacity of pDCs was determined as well as their potential to induce CD4(+) T-cell proliferation.RESULTS:
PDCs matured under Th1-like conditions showed a higher expression of antigens involved in T-cell co-stimulation and antigen presentation like CD40, CD80, CD83 and HLA-DR as well as a higher secretion of IL-6 and IFN-α in response to TLR7-ligation compared to Th2-pDCs. Furthermore, Th1-pDCs induced a significantly higher CD4(+) T-cell proliferation and primed a higher percentage of CD4(+) T cells to express IFN-γ and IL-2 after TLR7-ligation compared to Th2-pDCs. In contrast, Th2-pDCs were characterized by a significant upregulation of BDCA-3 and IL-4 expression following TLR7-ligation.CONCLUSION:
This study is the first to demonstrate the crucial impact of a surrounding cytokine environment on the development of pDC function including antigen expression. Based on these findings, it can be speculated that antiviral/bacterial pDC functions could be impaired during acute allergic conditions.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Dendríticas
/
Diferenciación Celular
/
Citocinas
/
Células Th2
/
Células TH1
Límite:
Humans
Idioma:
En
Revista:
Allergy
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania