Plasma alpha-galactosidase A:properties and comparisons with tissue alpha-galactosidases.
Biochim Biophys Acta
; 525(2): 399-409, 1978 Aug 07.
Article
en En
| MEDLINE
| ID: mdl-210818
ABSTRACT
The human plasma form of alpha-galactosidase A (alpha-D-galactoside galactohydrolase, EC 3.2.1.22) was highly purified and exhibited apparent Km values of 1.9 mM with 4-methylumbelliferyl-alpha-D-galactopyranoside and 0.23 mM with globotriglycosylceramide. Its inhibition with myo-inositol (Ki = 0.29 M) was similar to that observed with alpha-galactosidase A from various tissues. The plasma form of this lysosomal enzyme has a lower molecular weight of 96 600, a lower pI of 3.7 and faster electrophoretic mobility in polyacrylamide gels than the enzyme obtained from human liver. These data and the increased pI obtained after neuraminidase treatment suggest that the plasma form is an isoenzyme with a more highly sialylated carbohydrate moiety than the tissue isoenzymes.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Alfa-Galactosidasa
/
Galactosidasas
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1978
Tipo del documento:
Article