[Analysis of mitochondrial DNA D-loop region mutation and its significance in human oncocytoma].
Zhonghua Zhong Liu Za Zhi
; 32(10): 767-70, 2010 Oct.
Article
en Zh
| MEDLINE
| ID: mdl-21163068
ABSTRACT
OBJECTIVE:
To investigate the mutation in mitochondrial DNA displacement-loop (mtDNA D-loop) region in oncocytoma and its relationship with tumorigenesis and tumor development.METHODS:
The mtDNA D-Loop region of 20 thyroid or renal oncocytomas and the adjacent normal tissues were amplified by PCR, and then sequenced. Five human fetal renal tissues were collected as matched controls.RESULTS:
Among the 20 oncocytomas, 21 mutations which focused on hypervariable region I (HVI) were found in 7 tumor tissues and 1 normal tissue with the mutation rates of 35% and 5%, respectively. At the same time, 191 polymorphisms were found in the 20 cases.CONCLUSION:
mtDNA D-loop region, especially HV I, is the mutational hotspot of oncocytomas, which may be closely related with mtDNA duplicating rate and the function of mitochondria.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
ADN Mitocondrial
/
Neoplasias de la Tiroides
/
Adenoma Oxifílico
/
Neoplasias Renales
/
Mutación
Límite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Zhong Liu Za Zhi
Año:
2010
Tipo del documento:
Article
País de afiliación:
China