The NOD2 single nucleotide polymorphisms rs2066843 and rs2076756 are novel and common Crohn's disease susceptibility gene variants.
PLoS One
; 5(12): e14466, 2010 Dec 30.
Article
en En
| MEDLINE
| ID: mdl-21209938
ABSTRACT
BACKGROUND:
The aims were to analyze two novel NOD2 variants (rs2066843 and rs2076756) in a large cohort of patients with inflammatory bowel disease and to elucidate phenotypic consequences. METHODOLOGY/PRINCIPALFINDINGS:
Genomic DNA from 2700 Caucasians including 812 patients with Crohn's disease (CD), 442 patients with ulcerative colitis (UC), and 1446 healthy controls was analyzed for the NOD2 SNPs rs2066843 and rs2076756 and the three main CD-associated NOD2 variants p.Arg702Trp (rs2066844), p.Gly908Arg (rs2066847), and p.Leu1007fsX1008 (rs2066847). Haplotype and genotype-phenotype analyses were performed. The SNPs rs2066843 (pâ=â3.01×10(-5), OR 1.48, [95% CI 1.23-1.78]) and rs2076756 (pâ=â4.01×10(-6); OR 1.54, [95% CI 1.28-1.86]) were significantly associated with CD but not with UC susceptibility. Haplotype analysis revealed a number of significant associations with CD susceptibility with omnibus p values <10(-10). The SNPs rs2066843 and rs2076756 were in linkage disequilibrium with each other and with the three main CD-associated NOD2 mutations (D'>0.9). However, in CD, SNPs rs2066843 and rs2076756 were more frequently observed than the other three common NOD2 mutations (minor allele frequencies for rs2066843 and rs2076756 0.390 and 0.380, respectively). In CD patients homozygous for these novel NOD2 variants, genotype-phenotype analysis revealed higher rates of a penetrating phenotype (rs2076756 pâ=â0.015) and fistulas (rs2076756 pâ=â0.015) and significant associations with CD-related surgery (rs2076756 pâ=â0.003; rs2066843 pâ=â0.015). However, in multivariate analysis only disease localization (p<2×10(-16)) and behaviour (pâ=â0.02) were significantly associated with the need for surgery. CONCLUSION/SIGNIFICANCE:
The NOD2 variants rs2066843 and rs2076756 are novel and common CD susceptibility gene variants.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Variación Genética
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Enfermedad de Crohn
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Predisposición Genética a la Enfermedad
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Polimorfismo de Nucleótido Simple
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Proteína Adaptadora de Señalización NOD2
Tipo de estudio:
Etiology_studies
/
Incidence_studies
/
Observational_studies
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Risk_factors_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
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Child
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Humans
/
Middle aged
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Alemania