Extent of hematopoietic involvement by TET2 mutations in JAK2V6¹7F polycythemia vera.
Haematologica
; 96(5): 775-8, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21273266
ABSTRACT
TET2 mutations are found in polycythemia vera and it was initially reported that there is a greater TET2 mutational burden than JAK2(V617F) in polycythemia vera stem cells and that TET2 mutations precede JAK2(V617F). We quantified the proportion of TET2, JAK2(V617F) mutations and X-chromosome allelic usage in polycythemia vera cells, BFU-Es and in vitro expanded erythroid progenitors and found clonal reticulocytes, granulocytes, platelets and CD34(+) cells. We found that TET2 mutations may also follow rather than precede JAK2(V617F) as recently reported by others. Only a fraction of clonal early hematopoietic precursors and largely polyclonal T cells carry the TET2 mutation. We showed that in vitro the concomitant presence of JAK2(V617F) and TET2 mutations favors clonal polycythemia vera erythroid progenitors in contrast with non-TET2 mutated progenitors. We conclude that loss-of-function TET2 mutations are not the polycythemia vera initiating events and that the acquisition of TET2 somatic mutations may increase the aggressivity of the polycythemia vera clone.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Policitemia Vera
/
Proteínas Proto-Oncogénicas
/
Proteínas de Unión al ADN
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Janus Quinasa 2
/
Mutación
Límite:
Female
/
Humans
Idioma:
En
Revista:
Haematologica
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos