A noninvasive, direct real-time PCR method for sex determination in multiple avian species.
Mol Ecol Resour
; 11(2): 415-7, 2011 Mar.
Article
en En
| MEDLINE
| ID: mdl-21429156
ABSTRACT
Polymerase chain reaction (PCR)-based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high-throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high-throughput real-time PCR-based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Análisis para Determinación del Sexo
/
Aves
/
Reacción en Cadena de la Polimerasa
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Mol Ecol Resour
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos