[Inhibition of HIV-1 mediated cell-cell fusion by saponin fraction from Psidium guajava leaf].
Zhong Yao Cai
; 33(11): 1751-4, 2010 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-21434438
ABSTRACT
OBJECTIVE:
To investigate the effects of the total saponin of Psidium guajava leaf (TSGL) on HIV-1 envelop proteins (env) mediated virus entry into target cells.METHODS:
The TSGL was purified and concentrated using SA-1 macropore resin. The effect of TSGL on HIV-1 entry into target cells was tested using a cell-cell fusion assay by mixing CHO-WT and MT-2 cells. The cytotoxicity of TSGL was measured by MTT assay. The activity of TSGL on blocking the HIV-1 gp41 six helical bundle (6-HB) formation was analyzed by ELISA and Native-PAGE (N-PAGE).RESULTS:
The TSGL could inhibit HIV env mediated cell-cell fusion with an IC50 of (7.33 +/- 0.40) microg/mL, and displayed little cytotoxicity at that concentration. ELISA assay showed that the TSGL could prevent gp41 6-HB formation with inhibitory activity of 95.93% at 25 microg/mL. N-PAGE study confirmed the inhibitory effect of TSGL on gp41 6-HB formation.CONCLUSIONS:
The TSGL can inhibit HIV entry target cells by interfering the envelop subunit gp41 form the critical 6-HB structure.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saponinas
/
Proteína gp41 de Envoltorio del VIH
/
VIH-1
/
Inhibidores de Fusión de VIH
/
Psidium
Límite:
Humans
Idioma:
Zh
Revista:
Zhong Yao Cai
Asunto de la revista:
TERAPIAS COMPLEMENTARES
Año:
2010
Tipo del documento:
Article
País de afiliación:
China