Location of phosphoglycerate mutase in rat skeletal muscle. An immunocytochemical and biochemical study.
Eur J Cell Biol
; 51(1): 151-6, 1990 Feb.
Article
en En
| MEDLINE
| ID: mdl-2158448
ABSTRACT
The subcellular distribution of phosphoglycerate mutase was studied by immunogold techniques. With the aid of highly affinity-purified anti-phosphoglycerate mutase antibodies, the enzyme was found in both cytosol and nucleus of rat skeletal muscle. No evidence of interaction with contractile proteins was observed in cytosol. Nuclear location was also confirmed biochemically using purified nuclear preparations from rat skeletal muscle. Only one immunoreactive nuclear band was observed by Western blot experiments and corresponded to that of phosphoglycerate mutase mobility. Activity measurements from nuclear extracts showed that 25% of total specific activity is found in the nuclei.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfotransferasas
/
Fosfoglicerato Mutasa
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
Eur J Cell Biol
Año:
1990
Tipo del documento:
Article
País de afiliación:
España