Directed evolution of an error-prone T7 DNA polymerase that attenuates viral replication.
Chembiochem
; 12(10): 1551-8, 2011 Jul 04.
Article
en En
| MEDLINE
| ID: mdl-21626635
ABSTRACT
Experimental evidence exists that RNA viruses replicate with extremely high mutation rates that result in significant genetic diversity. The diverse nature of viral populations allows rapid adaptation to dynamic environments, and evolution of resistances to vaccines as well as antiviral substances. For DNA viruses that replicate at much greater fidelities, as yet, neither diverse structures in the population nor their responses to increased mutation rates have been sufficiently described. By using the example of DNA bacteriophage T7, we describe the identification of virus-specific DNA polymerase variants with decreased replication fidelities, and their impact on the efficiency of the viral infection cycle.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Replicación Viral
/
Bacteriófago T7
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Evolución Molecular Dirigida
/
ADN Polimerasa Dirigida por ADN
Idioma:
En
Revista:
Chembiochem
Asunto de la revista:
BIOQUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania