Differentiation of brown adipose tissue and biogenesis of thermogenic mitochondria in situ and in cell culture.
Biochim Biophys Acta
; 1018(2-3): 243-7, 1990 Jul 25.
Article
en En
| MEDLINE
| ID: mdl-2168211
ABSTRACT
Differentiation and biogenesis of mitochondria in brown adipose tissue (BAT) was studied in situ and in cell culture by Western blotting, enzyme activity measurements, [35S]methionine incorporation and immunofluorescence microscopy. In different rodent species the perinatal development of BAT thermogenic function resulted from the formation of thermogenic mitochondria which replaced the preexisting nonthermogenic mitochondria. Their biogenesis was characterized by the sudden appearance and rapid increase of the uncoupling protein (UCP), increase of cytochrome oxidase (COX) and decrease of H(+)-ATPase. In primary cell culture, differentiation of precursor cells from mouse BAT to typical multilocular adipocytes was accompanied by increasing content of COX and H(+)-ATPase. A selective synthesis of UCP was induced by activation of beta-adrenergic receptors or by elevated levels of cellular cAMP. UCP was quantitatively incorporated into mitochondria and within 24 h after stimulation reached near physiological concentration. Both in situ and in cell culture, the conditions enabling the expression of UCP gene were accompanied by activation of intracellular thyroxine 5'-deiodinase.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Desacopladores
/
Tejido Adiposo Pardo
/
ATPasas de Translocación de Protón
/
Complejo IV de Transporte de Electrones
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1990
Tipo del documento:
Article