Rapid identification of cyclic tetrapyrrolic photosensitisers for photodynamic therapy using on-line hyphenated LC-PDA-MS coupled with photo-cytotoxicity assay.
Phytochem Anal
; 23(1): 52-9, 2012.
Article
en En
| MEDLINE
| ID: mdl-21692117
ABSTRACT
INTRODUCTION:
Photodynamic therapy is a treatment modality that involves site-directed generation of cytotoxic reactive oxygen species by light-activated photosensitisers.OBJECTIVE:
In order to rapidly identify new photosensitisers from natural extracts, we developed a liquid chromatography-photodiode array-mass spectrometry (LC-PDA-MS) method to rapidly identify plant extracts that contain photosensitisers, particularly those possessing a cyclic tetrapyrrole structure.METHOD:
Six previously isolated compounds (1-6) were identified in bioactive fractions derived from 15 plant extracts on the basis of their chromatographic retention times, UV-visible profiles, accurate mass and fragmentation patterns.RESULTS:
Samples containing uncommon photosensitisers were rapidly identified using this method, and subsequent scale-up isolation efforts led to two new compounds (7 and 8) which were confirmed to be active photosensitisers in a photo-cytotoxicity assay.CONCLUSION:
This method serves as a useful tool in prioritising samples that may contain new photosensitisers out of a larger group of photo-cytotoxic natural products extracts.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plantas
/
Espectrometría de Masas
/
Extractos Vegetales
/
Cromatografía Liquida
/
Fármacos Fotosensibilizantes
/
Tetrapirroles
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Phytochem Anal
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Malasia