Multiple-locus variable-nucleotide tandem repeat subtype analysis implicates European starlings as biological vectors for Escherichia coli O157:H7 in Ohio, USA.
J Appl Microbiol
; 111(4): 982-8, 2011 Oct.
Article
en En
| MEDLINE
| ID: mdl-21762472
ABSTRACT
AIMS:
To provide molecular epidemiological evidence of avian transmission of Escherichia coli O157H7 between dairy farms in Ohio, this study was designed to identify genetic relatedness between isolates originating from bovine faecal samples and intestinal contents of European starlings captured on these farms. METHODS ANDRESULTS:
During a three-year period (2007-2009), cattle (n = 9000) and starlings (n = 430) on 150 different dairy farms in northern Ohio were sampled for the presence of E. coli O157H7. Isolates were subjected to multiple-locus variable-nucleotide tandem repeat analysis (MLVA). Distinct allelic groups were identified on most farms; however, isolates clustering into three MLVA groups originated from both cattle and birds on different farms.CONCLUSIONS:
Sharing of indistinguishable epidemiologically linked E. coli O157 MLVA subtypes between starlings and cattle on different farms supports the hypothesis that these birds contribute to the transmission of E. coli O157H7 between dairy farms. SIGNIFICANCE AND IMPACT OF STUDY A continued need exists to identify and to improve preharvest measures for controlling E. coli O157H7. Controlling wildlife intrusion, particularly European starlings, on livestock operations, may be an important strategy for reducing dissemination of E. coli O157H7 between farms and thereby potentially decreasing the on-farm prevalence of E. coli O157H7 and enhancing the safety of the food supply.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de los Bovinos
/
Escherichia coli O157
/
Estorninos
/
Vectores de Enfermedades
/
Infecciones por Escherichia coli
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
America do norte
Idioma:
En
Revista:
J Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos