Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion.
Cell Tissue Res
; 346(2): 237-44, 2011 Nov.
Article
en En
| MEDLINE
| ID: mdl-22009293
ABSTRACT
Lectins constitute a class of glycoproteins, which are capable of selectively and reversibly binding to carbohydrates, distinguishing small structural differences in complex oligosaccharides. Studies have shown that the binding of lectins to cell-surface carbohydrates can lead to various effects such as cellular proliferation, histamine release and cytokine production. Canavalia brasiliensis lectin (ConBr) is a (D-mannose) D-glucose lectin. In this study, murine splenocytes were cultured to determine the effect of ConBr on cell proliferation, nitric oxide (NO) release and cytokine secretion. In addition, cellular viability assays were performed to evaluate any mitogenic activity induced by this lectin. ConBr significantly increased cell proliferation with minimal cell damage. This lectin was able to induce an increased production of cytokines such as IL-2, IL-6 and IFN-γ and a decreased production of IL- 10. The release of NO was also observed. The results of this study indicate that ConBr could potentially be used as an immunomodulator.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Bazo
/
Adyuvantes Inmunológicos
/
Citocinas
/
Inmunización
/
Lectinas de Plantas
Límite:
Animals
Idioma:
En
Revista:
Cell Tissue Res
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil