Exploring the cell uptake mechanism of phospholipid and polyethylene glycol coated gold nanoparticles.
Nanotechnology
; 23(4): 045103, 2012 Feb 03.
Article
en En
| MEDLINE
| ID: mdl-22222168
ABSTRACT
Recently, there has been a lot of interest in using gold nanoparticles (GNPs) for biomedical applications due to their biocompatibility. To increase GNP cell uptake and circulation half-life, and to improve its bio-distribution in vivo, we chose to coat GNPs with 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (POPG) and polyethylene glycol (PEG). Two different methods were used to synthesize POPG-GNPs or PEG-GNPs, but the resulting nanoparticle sizes and morphologies were similar. Under the same incubation conditions, POPG-GNPs can be uptaken quicker than PEG-GNPs by cells-specifically, the maximum uptake was 8 h versus 16 h after incubation. In addition, the uptake amount of POPG-GNPs was more than that of PEG-GNPs. The uptake processes were confirmed by SEM and TEM images. The main reason for the greater uptake of POPG-GNPs can be attributed to the structural similarities between the POPG coating and the cell membrane as well as GNP aggregation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fosfatidilgliceroles
/
Polietilenglicoles
/
Endocitosis
/
Nanopartículas del Metal
/
Oro
Límite:
Humans
Idioma:
En
Revista:
Nanotechnology
Año:
2012
Tipo del documento:
Article
País de afiliación:
Canadá