Preimplantation genetic diagnosis for fetal neonatal alloimmune thrombocytopenia due to antihuman platelet antigen maternal antibodies.
Obstet Gynecol
; 119(2 Pt 1): 338-43, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-22270286
ABSTRACT
OBJECTIVE:
To develop a reliable preimplantation genetic diagnosis protocol for antihuman platelet antigen-1 incompatibility for a family in whom antenatal treatment was not possible because of the mother's hypersensitivity to intravenous immunoglobulin (IVIG).METHODS:
Haplotypes were constructed from genomic DNA of the family members. A polymerase chain reaction protocol that included eight microsatellite polymorphic markers and the ITGB3-specific (T196C, rs5918) polymorphism were multiplexed to be used in a single cell protocol, and single blastomeres were analyzed.RESULTS:
In one preimplantation genetic diagnosis cycle, out of 28 retrieved oocytes, 24 embryos fertilized and 12 underwent biopsy. Three embryos were found to be antihuman platelet antigen-1b/1b homozygotes and two were transferred. This cycle resulted in an uneventful pregnancy and birth of a healthy child.CONCLUSION:
In cases in which there is antihuman platelet antigen incompatibility and IVIG cannot be administered, preimplantation genetic diagnosis is a reliable alternative to enable birth of unaffected children.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pruebas Genéticas
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Antígenos de Plaqueta Humana
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Diagnóstico Preimplantación
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Integrina beta3
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Trombocitopenia Neonatal Aloinmune
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Hipersensibilidad
Tipo de estudio:
Diagnostic_studies
/
Guideline
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Prognostic_studies
Límite:
Adult
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Female
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Humans
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Male
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Newborn
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Pregnancy
Idioma:
En
Revista:
Obstet Gynecol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Israel