Solution structure of the natively assembled yeast ribosomal stalk determined by small-angle X-ray scattering.
Biochem J
; 444(2): 205-9, 2012 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-22458705
ABSTRACT
The ribosomal stalk of the 60S subunit has been shown to play a crucial role in all steps of protein synthesis, but its structure and exact molecular function remain an unanswered question. In the present study, we show the low-resolution models of the solution structure of the yeast ribosomal stalk, composed of five proteins, P0-(P1-P2)(2). The model of the pentameric stalk complex determined by small-angle X-ray scattering reveals an elongated shape with a maximum length of 13 nm. The model displays three distinct lobes, which may correspond to the individual P1-P2 heterodimers anchored to the C-terminal domain of the P0 protein.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Ribosómicas
/
Difracción de Rayos X
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Proteínas de Saccharomyces cerevisiae
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Dispersión del Ángulo Pequeño
Idioma:
En
Revista:
Biochem J
Año:
2012
Tipo del documento:
Article
País de afiliación:
Polonia