Digital polymerase chain reaction in an array of femtoliter polydimethylsiloxane microreactors.
Anal Chem
; 84(10): 4262-6, 2012 May 15.
Article
en En
| MEDLINE
| ID: mdl-22482776
ABSTRACT
We developed a simple, compact microfluidic device to perform high dynamic-range digital polymerase chain reaction (dPCR) in an array of isolated 36-femtoliter microreactors. The density of the microreactors exceeded 20000/mm(2). This device, made from polydimethylsiloxane (PDMS), allows the samples to be loaded into all microreactors simultaneously. The microreactors are completely sealed through the deformation of a PDMS membrane. The small volume of the microreactors ensures a compact device with high reaction efficiency and low reagent and sample consumption. Future potential applications of this platform include multicolor dPCR and massively parallel dPCR for next generation sequencing library preparation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Reacción en Cadena de la Polimerasa
/
Técnicas Analíticas Microfluídicas
/
Dimetilpolisiloxanos
Límite:
Animals
Idioma:
En
Revista:
Anal Chem
Año:
2012
Tipo del documento:
Article