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Differential diffusivity of Nodal and Lefty underlies a reaction-diffusion patterning system.
Müller, Patrick; Rogers, Katherine W; Jordan, Ben M; Lee, Joon S; Robson, Drew; Ramanathan, Sharad; Schier, Alexander F.
Afiliación
  • Müller P; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. pmueller@fas.harvard.edu
Science ; 336(6082): 721-4, 2012 May 11.
Article en En | MEDLINE | ID: mdl-22499809
Biological systems involving short-range activators and long-range inhibitors can generate complex patterns. Reaction-diffusion models postulate that differences in signaling range are caused by differential diffusivity of inhibitor and activator. Other models suggest that differential clearance underlies different signaling ranges. To test these models, we measured the biophysical properties of the Nodal/Lefty activator/inhibitor system during zebrafish embryogenesis. Analysis of Nodal and Lefty gradients revealed that Nodals have a shorter range than Lefty proteins. Pulse-labeling analysis indicated that Nodals and Leftys have similar clearance kinetics, whereas fluorescence recovery assays revealed that Leftys have a higher effective diffusion coefficient than Nodals. These results indicate that differential diffusivity is the major determinant of the differences in Nodal/Lefty range and provide biophysical support for reaction-diffusion models of activator/inhibitor-mediated patterning.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pez Cebra / Tipificación del Cuerpo / Proteínas de Pez Cebra / Blástula / Péptidos y Proteínas de Señalización Intracelular / Ligandos de Señalización Nodal / Factores de Determinación Derecha-Izquierda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Science Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Pez Cebra / Tipificación del Cuerpo / Proteínas de Pez Cebra / Blástula / Péptidos y Proteínas de Señalización Intracelular / Ligandos de Señalización Nodal / Factores de Determinación Derecha-Izquierda Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Science Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos