Luminescent iridium(III) complexes as novel protein staining agents.
Electrophoresis
; 33(9-10): 1397-401, 2012 May.
Article
en En
| MEDLINE
| ID: mdl-22648806
ABSTRACT
This article reports a new class of luminescent metal complexes, biscyclometalated iridium(III) complexes with an ancillary bathophenanthroline disulfonate ligand, for staining protein bands that are separated by electrophoresis. The performances of these novel staining agents have been studied in comparison with tris(bathophenanthroline disulfonate) ruthenium(II) tetrasodium salt (i.e. RuBPS) using a commercially available imaging system. The staining agents showed different limits of detection, linear dynamic ranges, and protein-to-protein variations. The overall performances of all three stains were found to be better than or equivalent to RuBPS under the experimental conditions.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fenantrolinas
/
Proteínas
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Complejos de Coordinación
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Colorantes Fluorescentes
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Iridio
Idioma:
En
Revista:
Electrophoresis
Año:
2012
Tipo del documento:
Article