[Expression of caspase-8 in non-small cell lung cancer and its role in invasion and metastasis of lung cancer A549 cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 28(6): 572-5, 2012 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-22691345
ABSTRACT
AIM:
To explore the expression of caspase-8 in non-small cell lung cancer (NSCLC) tissue, and its role in invasion and metastasis of lung cancer A549 cells.METHODS:
The expression of caspase-8 in the lung cancer tissues was detected by immunohistochemistry. RNA interference technology was used to silence the expression of caspase-8 in lung cancer A549 cells to explore the function of caspase-8 protein in invasion and metastasis of cancer cells by means of Western blot, Transwell and scratch migration assays.RESULTS:
The expression of caspase-8 was found in 38/52 cancer tissues, 22/30 adjacent tissues and 34/52 normal tissues. The tissues number of caspase-8 positive-expression in different groups was similar, but the intensity of caspase-8 expression in different groups was statistically different(P<0.05). In cancer tissues, the expression of caspase-8 indicated more likely to have lymph node-metastasis and worse clinical stage(P<0.05). After RNA interference, we successfully silenced the expression of caspase-8 in the lung cancer A549 cell, and this was confirmed by Western blotting. Transwell and cell scratch test showed that without the expression of caspase-8 A549 cells had a lower invasion and metastasis abilities compared to the control cells (P<0.05).CONCLUSION:
Caspase-8 is not only an apoptosis protein, but has non-apoptosis function, such as promoting invasion and metastasis of lung cancer A549 cell. In NSCLC, it more likely locates in nucleus. And its expression in NSCLC may indicate a later clinical stage and a higher probability of lymph node metastasis.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Carcinoma de Pulmón de Células no Pequeñas
/
Caspasa 8
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Neoplasias Pulmonares
Límite:
Adult
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Aged
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Female
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Humans
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Male
/
Middle aged
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China