Assembly of NADPH: protochlorophyllide oxidoreductase complex is needed for effective greening of barley seedlings.
J Plant Physiol
; 169(13): 1311-6, 2012 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-22704664
ABSTRACT
NADPHprotochlorophyllide (Pchlide) oxidoreductase (POR) is the key enzyme in the light-induced greening of higher plants. A unique light-harvesting PORPchlide complexes (LHPP) has been found in barley etioplasts, but not in other plant species. Why PORs from barley, but not from other plants, can form LHPP? And its function is not well understood. We modeled the barley and Arabidopsis POR proteins and compared molecular surface. The results confirm the idea that barley PORA can form a five-unit oligomer that interacts with a single PORB. Chemical treatment experiments indicated that POR complex may be formed by dithiol oxidation of cysteines of two adjacent proteins. We further showed that LHPP assembly was needed for barley POR functions and seedling greening. On the contrary, Arabidopsis POR proteins only formed dimers, which were not related to the functions or the greening. Finally, POR complex assembly (including LHPP and POR dimers) did not affect the formation of prolamellar bodies (PLBs) that function for efficient capture of light energy for photo conversion in etioplasts.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oxidorreductasas
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Protoclorofilida
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Hordeum
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Proteínas de Arabidopsis
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Plantones
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Complejos de Proteína Captadores de Luz
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NADP
Idioma:
En
Revista:
J Plant Physiol
Asunto de la revista:
BOTANICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
China