Multi-color miniature dual-axis confocal microscope for point-of-care pathology.
Opt Lett
; 37(12): 2430-2, 2012 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-22739931
ABSTRACT
We present a miniature microelectromechanical systems-based dual-axis confocal microscope capable of spatially coregistered fluorescence and reflectance imaging at multiple wavelengths. This device has a 10 mm diameter scan head with a 2 mm diameter tip for convenient use during surgery to guide tumor resection. The microscope has an adjustable focal depth of 20-200 micrometers and is capable of imaging with an axial resolution of 9 micrometers and in-plane resolution of 4 micrometers over a field of view of 450×450 micrometers. Simultaneous two-color imaging of individual optical sections is achieved by using a pair of grating-prism assemblies to compensate for chromatic dispersion in the 2 mm diameter gradient index relay lens at the distal tip of the device. Experimental measurements of the axial response of the microscope, as well as two-color images of a reflective bar target and fresh mouse brain tissues, demonstrate the performance of our device and its potential for multicolor in vivo optical sectioning microscopy.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Patología
/
Microscopía Confocal
/
Sistemas de Atención de Punto
/
Microtecnología
Límite:
Animals
Idioma:
En
Revista:
Opt Lett
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos