Natural Leishmania infection of Lutzomyia auraensis in Madre de Dios, Peru, detected by a fluorescence resonance energy transfer-based real-time polymerase chain reaction.
Am J Trop Med Hyg
; 87(3): 511-7, 2012 Sep.
Article
en En
| MEDLINE
| ID: mdl-22802444
ABSTRACT
Leishmania species of the Viannia subgenus are responsible for most cases of New World tegumentary leishmaniasis. However, little is known about the vectors involved in disease transmission in the Amazon regions of Peru. We used a novel real-time polymerase chain reaction (PCR) to assess Leishmania infections in phlebotomines collected in rural areas of Madre de Dios, Peru. A total of 1,299 non-blood fed female sand flies from 33 species were captured by using miniature CDC light traps. Lutzomyia auraensis was the most abundant species (63%) in this area. Seven of 164 pools were positive by PCR for Leishmania by kinetoplast DNA. The real-time PCR identified four Lu. auraensis pools as positive for L. (Viannia) lainsoni and L. (V.) braziliensis. The minimum infection prevalence for Lu. auraensis was estimated to be 0.6% (95% confidence interval = 0.20-1.42%). Further studies are needed to assess the importance of Lu. auraensis in the transmission of New World tegumentary leishmaniasis in hyperendemic areas of Peru.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Psychodidae
/
Leishmaniasis
/
ADN de Cinetoplasto
/
Transferencia Resonante de Energía de Fluorescencia
/
Reacción en Cadena en Tiempo Real de la Polimerasa
/
Insectos Vectores
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Peru
Idioma:
En
Revista:
Am J Trop Med Hyg
Año:
2012
Tipo del documento:
Article
País de afiliación:
Perú