Stability of lyophilized sucrose formulations of an IgG1: subvisible particle formation.
Pharm Dev Technol
; 18(4): 883-96, 2013.
Article
en En
| MEDLINE
| ID: mdl-22813478
ABSTRACT
Eight lyophilized formulations of a IgG1 monoclonal antibody (MAb) were prepared containing increasing levels of sucrose. In addition, three of the formulations had sorbitol added at a level of 5% w/w relative to sucrose. The samples were stored for up to 4 weeks at 40°C, which is well below the Tg. Upon reconstitution, the levels of subvisible particles were measured using microflow imaging (MFI). The formulation containing no sucrose contained exceedingly high levels of subvisible particles, accounting for as much as 25% of the weight of the protein. Addition of sucrose markedly decreased the number of subvisible particles, with the maximal sucroseprotein weight ratio being 21 (the highest level tested). Addition of sorbitol further decreased subvisible particle levels, even for formulations where the sucroseprotein ratio was relatively high. This suggests that even small amounts of a plasticizer like sorbitol can improve the storage stability of a lyophilized antibody formulation, probably by dampening ß-relaxations within the amorphous glass.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sacarosa
/
Inmunoglobulina G
/
Excipientes
/
Anticuerpos Monoclonales
Idioma:
En
Revista:
Pharm Dev Technol
Asunto de la revista:
FARMACIA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos