Molecular characterization of a new lytic bacteriophage isolated from cheese whey.
Arch Virol
; 157(12): 2265-72, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-22865166
ABSTRACT
In this study, we isolated and characterized a lytic Lactococcus lactis bacteriophage from the sera of a failed fermentation. The phage was isolated and cultured in L. lactis subsp. cremoris in M17 medium. The isolated bacteriophage was characterized by multiplex PCR, pulsed-field electrophoresis, DNA restriction digestion, analysis of the N-terminal sequence of the phage major structural protein, transmission electron microscopy and sequencing and analysis of a conserved fragment of its genome. Analysis of the viral genome indicates that its genome is composed of a DNA strand of approximately 48 kb in length, and PCR and microscopy confirmed that IL-P1 belongs to the group of 936-type phages in the family Siphoviridae, which is the most abundant type of lactococcal virus in dairy products worldwide. To our knowledge, this is the first report of a virus within this family that has a presumptive genome larger than 40 kb.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Queso
/
Lactococcus lactis
/
Siphoviridae
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil