Replication of hepatitis C virus genotype 3a in cultured cells.
Gastroenterology
; 144(1): 56-58.e7, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-22999961
ABSTRACT
Hepatitis C virus (HCV) genotype 3a is widespread worldwide, but no replication system exists for its study. We describe a subgenomic replicon system for HCV genotype 3a. We determined the consensus sequence of an HCV genome isolated from a patient, and constructed a subgenomic replicon using this clone. The replicon was transfected into HuH-7 cells and RNA replication was confirmed. We identified cell culture-adaptive mutations that increased colony formation multiple-fold. We have therefore established a genotype 3a replicon system that can be used to study this HCV genotype.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Replicón
/
Replicación Viral
/
ARN Viral
/
Hepacivirus
Límite:
Humans
Idioma:
En
Revista:
Gastroenterology
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón