The D3 F-box protein is a key component in host strigolactone responses essential for arbuscular mycorrhizal symbiosis.
New Phytol
; 196(4): 1208-1216, 2012 Dec.
Article
en En
| MEDLINE
| ID: mdl-23025475
ABSTRACT
Arbuscular mycorrhiza (AM) represents an ancient endosymbiosis between plant roots and Glomeromycota fungi. Strigolactones (SLs), plant-derived terpenoid lactones, activate hyphal branching of AM fungi before physical contact. Lack of SL biosynthesis results in lower colonization of AM fungi. The F-box protein, DWARF3 (D3), and the hydrolase family protein DWARF14 (D14) are crucial for SL responses in rice. Here we conducted AM fungal colonization assays with the SL-insensitive d3 and d14 mutants. The d3 mutant exhibited strong defects in AM fungal colonization, whereas the d14 mutant showed higher AM fungal colonization. As D14 has a homologous protein, D14-LIKE, we generated D14-LIKE knockdown lines by RNA interference in the wildtype and d14 background. D14 and D14-LIKE double knockdown lines exhibited similar colonization rates as those of the d14-1 mutant. D3 is crucial for establishing AM symbiosis in rice, whereas D14 and D14-LIKE are not. Our results suggest distinct roles for these SL-related components in AM symbiosis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Oryza
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Simbiosis
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Terpenos
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Micorrizas
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Proteínas F-Box
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Glomeromycota
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Lactonas
Idioma:
En
Revista:
New Phytol
Asunto de la revista:
BOTANICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Japón