Temporal classification and mapping of non-polyadenylated transcripts of an invertebrate iridovirus.
J Gen Virol
; 94(Pt 1): 187-192, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23052394
ABSTRACT
The temporal expression of the 54 Chilo iridescent virus (CIV) virion protein genes was investigated by combining drug treatments that inhibit protein or DNA synthesis and an RT-PCR strategy particularly suitable for non-polyadenylated mRNAs. This method generates a uniform 3' terminus by ligation of a 5'-phosphorylated oligonucleotide to the 3' end of the transcript that is recognized by a complementary primer during RT-PCR. This analysis showed that CIV virion proteins are encoded by genes in all three predetermined temporal classes 23 immediate-early, 11 delayed-early and seven late virion gene transcripts were identified and assigned to ORFs. Early transcription of many virion protein genes supports the notion that virion proteins may also play essential roles in the initial stages of infection. In addition, some of the early gene products present in the virion may reflect the intracellular path that the virus follows during infection.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Iridovirus
Límite:
Animals
Idioma:
En
Revista:
J Gen Virol
Año:
2013
Tipo del documento:
Article
País de afiliación:
Países Bajos