Assays for methionine γ-lyase and S-adenosyl-L-homocysteine hydrolase based on enzymatic formation of CdS quantum dots in situ.
Anal Chem
; 84(21): 8961-5, 2012 Nov 06.
Article
en En
| MEDLINE
| ID: mdl-23083214
ABSTRACT
S-Adenosyl-L-homocysteine hydrolase (AHCY) hydrolyzes its substrate S-adenosyl-L-homocysteine (AdoHcy) to L-homocysteine (Hcy). Methionine γ-lyase (MGL) catalyzes the decomposition of Hcy to hydrogen sulfide which forms fluorescent CdS nanoparticles in the presence of Cd(NO(3))(2). On the basis of these enzymatic reactions, two new simple and robust fluorogenic enzymatic assays for MGL and AHCY were developed and applied to detection of AHCY inhibitors.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Liasas de Carbono-Azufre
/
Sulfuros
/
Compuestos de Cadmio
/
Adenosilhomocisteinasa
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Puntos Cuánticos
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Pruebas de Enzimas
Idioma:
En
Revista:
Anal Chem
Año:
2012
Tipo del documento:
Article