Prandial ghrelin attenuation provides evidence that des-acyl ghrelin may be an artifact of sample handling in human plasma.
Bioanalysis
; 4(20): 2447-55, 2012 Oct.
Article
en En
| MEDLINE
| ID: mdl-23157354
ABSTRACT
BACKGROUND:
Plasma acyl and des-acyl ghrelin are thought of as components of total ghrelin, but this has never been validated using ex vivo spiking experiments, human sample collection comparisons and fit-for-purpose translatable assays.RESULTS:
Acyl ghrelin plasma stability was analyzed by LC-MS/MS and it revealed that acyl ghrelin is enzymatically and chemically converted to des-acyl ghrelin in the presence of active serine proteases and HCl. ELISAs with less than 30% total error were used to assess acyl ghrelin behavior in matched authentic human samples. Acyl and total ghrelin were not statistically different in 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride samples and acyl ghrelin losses in K(2)EDTA plasma were accounted for in des-acyl ghrelin formation.CONCLUSION:
Acyl ghrelin is total ghrelin and des-acyl ghrelin should not be detectible in healthy human plasma under optimal sample handling and assaying conditions.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Cromatografía Líquida de Alta Presión
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Espectrometría de Masas en Tándem
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Ghrelina
Límite:
Adult
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Bioanalysis
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos