Fast multicolor 3D imaging using aberration-corrected multifocus microscopy.
Nat Methods
; 10(1): 60-3, 2013 Jan.
Article
en En
| MEDLINE
| ID: mdl-23223154
ABSTRACT
Conventional acquisition of three-dimensional (3D) microscopy data requires sequential z scanning and is often too slow to capture biological events. We report an aberration-corrected multifocus microscopy method capable of producing an instant focal stack of nine 2D images. Appended to an epifluorescence microscope, the multifocus system enables high-resolution 3D imaging in multiple colors with single-molecule sensitivity, at speeds limited by the camera readout time of a single image.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Caenorhabditis elegans
/
Imagenología Tridimensional
/
Rastreo Celular
/
Microscopía Fluorescente
/
Neuronas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2013
Tipo del documento:
Article
País de afiliación:
Estados Unidos