Cloning, expression and characterization of a trehalose synthase gene from Rhodococcus opacus.
Protein J
; 32(3): 223-9, 2013 Mar.
Article
en En
| MEDLINE
| ID: mdl-23512410
ABSTRACT
Trehalose is a unique disaccharide capable of protecting proteins against environmental stress. A novel trehalose synthase (TreS) gene from Rhodococcus opacus was cloned and expressed in Escherichia coli Top10 and BL21 (DE3) pLysS, respectively. The recombinant TreS showed a molecular mass of 79 kDa. Thin layer chromatography (TLC) result suggested that this enzyme had the ability to catalyze the mutual conversion of maltose and trehalose. Moreover, high-performance liquid chromatography (HPLC) result suggested that glucose appeared as a byproduct with a conversion rate of 12 %. The purified recombinant enzyme had an optimum temperature of 25 °C and pH optimum around 7.0. Kinetic analysis revealed that the K m for trehalose was around 98 mM, which was a little higher than that of maltose. The preferred substrate of TreS was maltose according to the analysis of k cat/K m. Both 1 and 10 mM of Hg(2+), Cu(2+) and Al(3+) could inhibit the TreS activity, while only 1 mM of Ca(2+) and Mn(2+) could increase its activity. Five amino acid residues, Asp(244), Glu(286), Asp(354), His(147) and His(353), were shown to be conserved in R. opacus TreS, which were also important for α-amylase family enzyme catalysis.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Rhodococcus
/
Clonación Molecular
/
Glucosiltransferasas
Idioma:
En
Revista:
Protein J
Asunto de la revista:
BIOQUIMICA
Año:
2013
Tipo del documento:
Article
País de afiliación:
China