Production and diagnostic application of recombinant domain III of West Nile envelope protein in Brazil.
Rev Soc Bras Med Trop
; 46(1): 97-9, 2013.
Article
en En
| MEDLINE
| ID: mdl-23563834
ABSTRACT
INTRODUCTION:
West Nile virus (WNV) is a flavivirus with a natural cycle involving mosquitoes and birds. Over the last 11 years, WNV has spread throughout the Americas with the imminent risk of its introduction in Brazil.METHODS:
Envelope protein domain III of WNV (rDIII) was bacterially expressed and purified. An enzyme-linked immunosorbent assay with WNV rDIII antigen was standardized against mouse immune fluids (MIAFs) of different flavivirus.RESULTS:
WNV rDIII reacted strongly with St. Louis encephalitis virus (SLEV) MIAF but not with other flaviviruses.CONCLUSIONS:
This antigen may be a potentially useful tool for serologic diagnosis and may contribute in future epidemiological surveillance of WNV infections in Brazil.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Virus del Nilo Occidental
/
Proteínas Recombinantes
/
Proteínas del Envoltorio Viral
/
Anticuerpos Neutralizantes
/
Anticuerpos Antivirales
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Rev Soc Bras Med Trop
Año:
2013
Tipo del documento:
Article
País de afiliación:
Brasil