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Comparison of endogenous and overexpressed MyoD shows enhanced binding of physiologically bound sites.
Yao, Zizhen; Fong, Abraham P; Cao, Yi; Ruzzo, Walter L; Gentleman, Robert C; Tapscott, Stephen J.
Afiliación
  • Yao Z; Human Biology Division, Seattle, WA, USA.
  • Fong AP; Clinical Research Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, WA, 98109, USA.
  • Cao Y; Department of Pediatrics, University of Washington, School of Medicine, Seattle, WA, 98105, USA.
  • Ruzzo WL; Bioinformatics and Computational Biology, Genentech, South San Francisco, CA, USA.
  • Gentleman RC; Departments of Computer Science and Engineering and Genome Sciences, Seattle, WA, USA.
  • Tapscott SJ; Bioinformatics and Computational Biology, Genentech, South San Francisco, CA, USA.
Skelet Muscle ; 3(1): 8, 2013 Apr 08.
Article en En | MEDLINE | ID: mdl-23566431
BACKGROUND: Transcription factor overexpression is common in biological experiments and transcription factor amplification is associated with many cancers, yet few studies have directly compared the DNA-binding profiles of endogenous versus overexpressed transcription factors. METHODS: We analyzed MyoD ChIP-seq data from C2C12 mouse myotubes, primary mouse myotubes, and mouse fibroblasts differentiated into muscle cells by overexpression of MyoD and compared the genome-wide binding profiles and binding site characteristics of endogenous and overexpressed MyoD. RESULTS: Overexpressed MyoD bound to the same sites occupied by endogenous MyoD and possessed the same E-box sequence preference and co-factor site enrichments, and did not bind to new sites with distinct characteristics. CONCLUSIONS: Our data demonstrate a robust fidelity of transcription factor binding sites over a range of expression levels and that increased amounts of transcription factor increase the binding at physiologically bound sites.

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Skelet Muscle Año: 2013 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Skelet Muscle Año: 2013 Tipo del documento: Article País de afiliación: Estados Unidos