[Auto-vaccines: an immunological alternative to gene silencing]. / Auto-vaccins - Une alternative immunologique à l'inactivation génique.
Med Sci (Paris)
; 29(4): 425-9, 2013 Apr.
Article
en Fr
| MEDLINE
| ID: mdl-23621939
ABSTRACT
Auto-vaccination is a procedure that recently attracted the interest of a growing number of investigators as an alternative to gene inactivation for functional studies of cytokines or other mediators. It is based on the observation that autologous cytokines cross-linked to a foreign protein or peptide are recognized by self-reactive B cells that present foreign peptides, and by doing so attract illicit help from helper T cells that recognize the foreign peptide on the self-reactive B cell MHC Class II complex. This leads to the production of antibodies reacting with self-proteins and thus to neutralization of the targeted factor. Here, we summarize the different techniques that were successful in breaking this self-tolerance and provide several examples of the functional consequences of these auto-vaccines. An additional output of auto-vaccination is the production of mouse monoclonal antibodies against mouse factors. Such antibodies have obvious advantages for long-term use in vivo.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Silenciador del Gen
/
Autovacunas
Límite:
Animals
/
Humans
Idioma:
Fr
Revista:
Med Sci (Paris)
Asunto de la revista:
MEDICINA
Año:
2013
Tipo del documento:
Article
País de afiliación:
Bélgica