C6-Deoxy coelenterazine analogues as an efficient substrate for glow luminescence reaction of nanoKAZ: the mutated catalytic 19 kDa component of Oplophorus luciferase.
Biochem Biophys Res Commun
; 437(1): 23-8, 2013 Jul 19.
Article
en En
| MEDLINE
| ID: mdl-23792095
ABSTRACT
The codon-optimized gene for the mutated 19 kDa protein (nanoKAZ), which is the catalytic component of Oplophorus luciferase, was expressed in Escherichia coli cells and the recombinant protein was highly purified. The secretory expression of nanoKAZ from CHO-K1 cells was performed by fusing the secretory signal peptide sequence of Gaussia luciferase to the amino-terminus of nanoKAZ. The substrate specificity for the purified nanoKAZ and the nanoKAZ secreted into the cultured medium was determined, indicating that bis-coelenterazine (bis-CTZ) and newly synthesized 6h-f-coelenterazine (6h-f-CTZ) are an efficient substrate for the glow luminescence reaction of nanoKAZ.
Palabras clave
19kOLase; CTZ; Codon-optimized gene; Coelenterazine; FWHM; Furimazine; GLase; Gaussia; Gaussia luciferase; I(max); Reporter assay; coelenterazine; full width at half maximum; maximum intensity of luminescence; nanoKAZ; relative light units; rlu; the catalytic 19kDa protein of Oplophorus luciferase; the mutated 19kOLase
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pirazinas
/
Decápodos
/
Dominio Catalítico
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Luminiscencia
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Proteínas Mutantes
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Imidazoles
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Luciferasas
Límite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2013
Tipo del documento:
Article
País de afiliación:
Japón