Active plasma membrane P-type H+-ATPase reconstituted into nanodiscs is a monomer.
J Biol Chem
; 288(37): 26419-29, 2013 Sep 13.
Article
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| MEDLINE
| ID: mdl-23836891
ABSTRACT
Plasma membrane H(+)-ATPases form a subfamily of P-type ATPases responsible for pumping protons out of cells and are essential for establishing and maintaining the crucial transmembrane proton gradient in plants and fungi. Here, we report the reconstitution of the Arabidopsis thaliana plasma membrane H(+)-ATPase isoform 2 into soluble nanoscale lipid bilayers, also termed nanodiscs. Based on native gel analysis and cross-linking studies, the pump inserts into nanodiscs as a functional monomer. Insertion of the H(+)-ATPase into nanodiscs has the potential to enable structural and functional characterization using techniques normally applicable only for soluble proteins.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Membrana Celular
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Arabidopsis
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ATPasas de Translocación de Protón
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Proteínas de Arabidopsis
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Membrana Dobles de Lípidos
Idioma:
En
Revista:
J Biol Chem
Año:
2013
Tipo del documento:
Article