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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool†Dedicated to the memory of Joachim H. G. Steinke.‡Electronic supplementary information (ESI) available: Further experimental data. See DOI: 10.1039/c3sm00131hClick here for additional data file.
Piluso, Susanna; Cassell, Heather C; Gibbons, Jonathan L; Waller, Thomas E; Plant, Nick J; Miller, Aline F; Cavalli, Gabriel.
Afiliación
  • Piluso S; Department of Chemistry , University of Surrey , Guildford , GU2 7XH , UK . Email: g.cavalli@surrey.ac.uk ; Tel: +44 (0)1483 686837.
Soft Matter ; 9(29): 6752-6756, 2013 Aug 07.
Article en En | MEDLINE | ID: mdl-23847687
The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Soft Matter Año: 2013 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Soft Matter Año: 2013 Tipo del documento: Article