In situ sequencing for RNA analysis in preserved tissue and cells.
Nat Methods
; 10(9): 857-60, 2013 Sep.
Article
en En
| MEDLINE
| ID: mdl-23852452
ABSTRACT
Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Conservación de Tejido
/
Análisis de Secuencia de ARN
/
Perfilación de la Expresión Génica
Límite:
Female
/
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2013
Tipo del documento:
Article
País de afiliación:
Suecia