Colorantes Fluorescentes/síntesis química; Receptores de Melanocortina/metabolismo; Unión Competitiva; Colorantes Fluorescentes/química; Colorantes Fluorescentes/metabolismo; Células HEK293; Humanos; Ácido Pentético/química; Péptidos/síntesis química; Péptidos/química; Péptidos/metabolismo; Unión Proteica; Receptor de Colecistoquinina B/química; Receptor de Colecistoquinina B/genética; Receptor de Colecistoquinina B/metabolismo; Receptor de Melanocortina Tipo 4/química; Receptor de Melanocortina Tipo 4/genética; Receptor de Melanocortina Tipo 4/metabolismo; Receptores de Melanocortina/química; Técnicas de Síntesis en Fase Sólida

1-(9H-fluoren-9-yl)-3,19-dioxo-2,8,11,14,21-pentaoxa-4,18-diazatricosan-23-oic acid; 1-hydroxybenzotriazole; 19-amino-5-oxo-3,10,13,16-tetraoxa-6-azanonadecan-1-oic acid; 6-chloro-1-hydroxybenzotriazole; 9-fluorenylmethyoxycarbonyl; Ac-Ser-Tyr-Ser-Nle-Glu-His-DPhe-Arg-Trp-Gly-Lys-Pro-Val-NH(2); BSA; Cl-HOBt; Competition binding assays; CuAAC; DCM; DIC; DIEA; DMEM; DMF; DMSO; DTPA; Dulbecco's Modified Eagle Medium; ESI MS; FT-ICR MS; Fluorescent probes; Fmoc; Fmoc-PEGO; Fourier transform ion cyclotron resonance mass spectrometry; HOBt; HRMS; His-DPhe-Arg-Trp; IC(50); MEM; MSH(4); MSH(7); Melanocortin 4 receptor; Minimum Essential Medium; N,N-dimethylformamide; NDP-α-MSH; PEGO; Saturation binding assays; Ser-Nle-Glu-His-DPhe-Arg-Trp; TBTA; TEAA; TFA; THF; TLC; TRF; Time-resolved fluorescence; bovine serum albumin; copper(I)-catalyzed azide-alkyne cycloaddition; dichloromethane; diethylenetriaminepentaacetic acid; diisopropyl carbodiimide; diisopropylethylamine; dimethyl sulfoxide; electrospray ionization mass spectrometry; hMC4R; half maximal inhibitory concentration; high resolution mass spectroscopy; human melanocortin 4 receptor; tetrahydrofuran; thin-layer chromatography; time-resolved fluorescence; triethylammonium acetate; trifluoroacetic acid; tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine