A new tool improves diagnostic test performance for transmission em evaluation of axonemal dynein arms.
Ultrastruct Pathol
; 38(4): 248-55, 2014 Aug.
Article
en En
| MEDLINE
| ID: mdl-23957500
ABSTRACT
Abstract Diagnosis of primary ciliary dyskinesia (PCD) by identification of dynein arm loss in transmission electron microscopy (TEM) images can be confounded by high background noise due to random electron-dense material within the ciliary matrix, leading to diagnostic uncertainty even for experienced morphologists. The authors developed a novel image analysis tool to average the axonemal peripheral microtubular doublets, thereby increasing microtubular signal and reducing random background noise. In a randomized, double-blinded study that compared two experienced morphologists and three different diagnostic approaches, they found that use of this tool led to improvement in diagnostic TEM test performance.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Interpretación de Imagen Asistida por Computador
/
Síndrome de Kartagener
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Microscopía Electrónica de Transmisión
/
Dineínas Axonemales
Tipo de estudio:
Clinical_trials
/
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Ultrastruct Pathol
Año:
2014
Tipo del documento:
Article