Vascular endothelial growth factor receptor 1 expression in nasal polyp tissue.
Allergy
; 69(2): 237-45, 2014 Feb.
Article
en En
| MEDLINE
| ID: mdl-24127643
ABSTRACT
BACKGROUND:
Edema represents a key feature of nasal polyp (NP) disease. Members of the vascular endothelial growth factor (VEGF) family may be involved, but the precise role of VEGF-A, VEGF-B, placental growth factor (PlGF), and their receptors VEGFR1 and VEGFR2 in NP edema formation remains elusive.OBJECTIVE:
Exploring the expression of VEGF family members and their receptors and their correlation with clinical, radiological, and edema markers in NP.METHODS:
The expression of VEGF-A, VEGF-B, PlGF, VEGFR1, and VEGFR2 was measured in NP (n = 23) and control tissue (n = 22) at mRNA and protein level. Edema was evaluated by measuring albumin levels and wet/dry ratios. Computed tomography (CT) scans were scored using the Lund-Mackay scoring system. IL-5 mRNA expression was determined by real-time RT-PCR. Cell suspensions from NP (n = 10) and control tissue (n = 12) were stimulated in vitro with IL-1ß or TNFα.RESULTS:
mRNA expression of VEGFR1 and VEGF-B was significantly higher in NP compared with control tissue. Expression levels of VEGF-B and VEGFR1 significantly correlated with NP albumin content (VEGF-B P = 0.0208; VEGFR1 P = 0.0293), CT scan scores (VEGF-B P = 0.0075; VEGFR1 P = 0.0068), and IL-5 mRNA (VEGF-B P = 0.0027; VEGFR1 P = 0.0001). In vitro stimulation of control and NP tissue cell suspensions with IL-1ß or TNFα significantly reduced the expression of VEGFR2 in control tissue, without altering VEGFR1 and VEGF-B expression. hVEGF-B induced nitric oxide production in NP macrophages (P < 0.05).CONCLUSION:
Expression levels of VEGFR1 and VEGF-B correlate with edema and clinical markers of NP disease and therefore represent potential therapeutic targets.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pólipos Nasales
/
Receptor 1 de Factores de Crecimiento Endotelial Vascular
Límite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Allergy
Año:
2014
Tipo del documento:
Article
País de afiliación:
Bélgica