Monitoring dynamic interactions of tumor cells with tissue and immune cells in a lab-on-a-chip.
Anal Chem
; 85(23): 11471-8, 2013 Dec 03.
Article
en En
| MEDLINE
| ID: mdl-24215610
ABSTRACT
A complementary cell analysis method has been developed to assess the dynamic interactions of tumor cells with resident tissue and immune cells using optical light scattering and impedance sensing to shed light on tumor cell behavior. The combination of electroanalytical and optical biosensing technologies integrated in a lab-on-a-chip allows for continuous, label-free, and noninvasive probing of dynamic cell-to-cell interactions between adherent and nonadherent cocultures, thus providing real-time insights into tumor cell responses under physiologically relevant conditions. While the study of adherent cocultures is important for the understanding and suppression of metastatic invasion, the analysis of tumor cell interactions with nonadherent immune cells plays a vital role in cancer immunotherapy research. For the first time, the direct cell-to-cell interactions of tumor cells with bead-activated primary T cells were continuously assessed using an effector cell to target a cell ratio of 101.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Linfocitos T
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Comunicación Celular
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Dispositivos Laboratorio en un Chip
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Células Endoteliales de la Vena Umbilical Humana
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Fibroblastos
Límite:
Adult
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Anal Chem
Año:
2013
Tipo del documento:
Article
País de afiliación:
Austria