An ELISA method for quantification of Tamm-Horsfall protein using monoclonal antibodies.
J Immunol Methods
; 98(1): 105-11, 1987 Apr 02.
Article
en En
| MEDLINE
| ID: mdl-2435805
ABSTRACT
Eight hybridoma cell lines secreting monoclonal antibodies (MoAbs) to Tamm-Horsfall protein (THP) were established. The isotype and reaction pattern of the MoAbs with THP from rat, rabbit, guinea pig and man were employed for the selection of clones. At least four epitopes were recognised on human THP. One of these epitopes differed from the others in its dependence on the state of aggregation of the THP. An ELISA procedure was developed for quantification of THP in urine requiring no other sample treatment than dilution in the assay buffer. In this ELISA, THP showed an increased immunoreactivity after freezing.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ensayo de Inmunoadsorción Enzimática
/
Anticuerpos Monoclonales
/
Mucoproteínas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1987
Tipo del documento:
Article