Reprogramming of enteroendocrine K cells to pancreatic ß-cells through the combined expression of Nkx6.1 and Neurogenin3, and reaggregation in suspension culture.

Lee, Esder; Ryu, Gyeong Ryul; Moon, Sung-Dae; Ko, Seung-Hyun; Ahn, Yu-Bae; Song, Ki-Ho

Lee, Esder; Ryu, Gyeong Ryul; Moon, Sung-Dae; Ko, Seung-Hyun; Ahn, Yu-Bae; Song, Ki-Ho.

Afiliación

Lee E; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Ryu GR; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Moon SD; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Ko SH; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Ahn YB; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Song KH; Division of Endocrinology & Metabolism, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. Electronic address: kihos@catholic.ac.kr.

Biochem Biophys Res Commun ; 443(3): 1021-7, 2014 Jan 17.

Article en En | MEDLINE | ID: mdl-24365150

Asunto(s)

Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico/metabolismo; Técnicas de Cultivo de Célula/métodos; Reprogramación Celular; Células Enteroendocrinas/metabolismo; Proteínas de Homeodominio/metabolismo; Células Secretoras de Insulina/citología; Células Secretoras de Insulina/metabolismo; Proteínas del Tejido Nervioso/metabolismo; Animales; Agregación Celular; Células Enteroendocrinas/citología; Regulación de la Expresión Génica; Proteínas Fluorescentes Verdes/metabolismo; Células Secretoras de Insulina/ultraestructura; Ratones; Ratones Desnudos; Ratas; Suspensiones

Palabras clave

4',6'-diamidino-2-phenylindole; Adv; DAPI; DAPT; DMEM; Differentiation; Dulbecco's Modified Eagle's Medium; Enteroendocrine cells; GFP; GIP; GIPP; GLP-1; GLUT2; ITS; Islet; Islet transplantation; K cells; KRB; Krebs Ringer Buffer; MOI; N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester; NGN3; Neurogenin 3; PC; PDX1; Reprogramming; adenovirus; glucagon-like peptide-1; glucose transporter 2; glucose-dependent insulinotropic polypeptide; glucose-dependent insulinotropic polypeptide promoter; green fluorescent protein; insulin/transferring/sodium selenite; multiplicity of infection; pancreatic and duodenal homeobox-1; prohormone convertase; ß-Cells

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Proteínas de Homeodominio / Técnicas de Cultivo de Célula / Células Enteroendocrinas / Células Secretoras de Insulina / Factores de Transcripción con Motivo Hélice-Asa-Hélice Básico / Reprogramación Celular / Proteínas del Tejido Nervioso Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 2014 Tipo del documento: Article

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