Patterning and growth control by membrane-tethered Wingless.
Nature
; 505(7482): 180-5, 2014 Jan 09.
Article
en En
| MEDLINE
| ID: mdl-24390349
ABSTRACT
Wnts are evolutionarily conserved secreted signalling proteins that, in various developmental contexts, spread from their site of synthesis to form a gradient and activate target-gene expression at a distance. However, the requirement for Wnts to spread has never been directly tested. Here we used genome engineering to replace the endogenous wingless gene, which encodes the main Drosophila Wnt, with one that expresses a membrane-tethered form of the protein. Surprisingly, the resulting flies were viable and produced normally patterned appendages of nearly the right size, albeit with a delay. We show that, in the prospective wing, prolonged wingless transcription followed by memory of earlier signalling allows persistent expression of relevant target genes. We suggest therefore that the spread of Wingless is dispensable for patterning and growth even though it probably contributes to increasing cell proliferation.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Membrana Celular
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Tipificación del Cuerpo
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Proteínas de Drosophila
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Drosophila melanogaster
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Proteína Wnt1
Límite:
Animals
Idioma:
En
Revista:
Nature
Año:
2014
Tipo del documento:
Article